NAD+ reconstitution calculator
NAD+ vials hold hundreds of milligrams, not the few milligrams of a typical peptide, so the numbers get large fast. Enter the vial size, the water you add and the amount in mg to see the concentration and the units on a U-100 insulin syringe.
This calculator is a neutral conversion tool (mg ↔ solvent ↔ units). It makes no recommendation as to whether, what or how much to use, and does not replace medical advice. All inputs are your own.
Why NAD+ needs its own numbers
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme, not a short peptide. A typical peptide vial holds a few milligrams; an NAD+ vial holds hundreds, and amounts are given in mg instead of mcg. That is why NAD+ is often dissolved in more water, and why one draw can take up a large part of a 1 mL syringe.
The maths stays the same: units = (amount in mg ÷ concentration in mg/mL) × 100. The table shows how the water volume changes the draw for a 500 mg vial and a 100 mg amount. At 5 mL, a single draw already fills a whole 100-unit syringe:
| Water added | Concentration | Units to draw |
|---|---|---|
| 2 mL | 250 mg/mL | 40 U |
| 3 mL | 166.67 mg/mL | 60 U |
| 4 mL | 125 mg/mL | 80 U |
| 5 mL | 100 mg/mL | 100 U |
Dissolved NAD+ is less stable than the dry powder: reagent datasheets describe a fresh solution as clear and colourless to at most faintly yellow and advise against keeping it for more than a day. What a yellow tint means, and what the research on NAD+ shows:
How to use the peptide calculator
- Enter the vial strength — the total milligrams of peptide in the vial.
- Enter the bacteriostatic (BAC) water you added, in millilitres.
- Enter your target dose in mcg or mg.
- Read the units to draw on a U-100 insulin syringe, plus the concentration and doses per vial.
Peptide reconstitution — frequently asked questions
How do I reconstitute a peptide?
Reconstitution means dissolving freeze-dried (lyophilized) peptide powder in bacteriostatic water. Add the water slowly down the vial wall and let it dissolve without shaking. Enter the vial strength, the BAC water you added and your target dose above, and the calculator shows the concentration and exactly how many units to draw.
How much bacteriostatic water should I add?
There is no single correct amount. More BAC water lowers the concentration and gives a larger, easier-to-read draw; less water raises the concentration and shrinks the draw. Choose an amount that keeps your dose within your syringe's range — change the BAC water value above to see the units update live.
How many units do I draw on an insulin syringe?
On a U-100 insulin syringe, 100 units equal 1 mL. The calculator converts your dose and concentration into units so you can read them straight off the syringe barrel. The figure above updates instantly as you change the inputs.
How do I convert mcg to units?
Units depend on the concentration, not the dose alone — the same dose in mcg draws to different units depending on how much BAC water you used. Enter your dose in mcg or mg with the vial and water values, and the calculator returns the exact units to draw.
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